Unraveling condition‐dependent networks of transcription factors that control metabolic pathway activity in yeast

Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast

Which transcription factors control the distribution of metabolic fluxes under a given condition? We address this question by systematically quantifying metabolic fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae under five growth conditions. While most knockouts did not affect fluxes, we identified 42 condition-dependent interactions that were mediated by a total ...

Metabolic Profiling of Retrograde Pathway Transcription Factors Rtg1 and Rtg3 Knockout Yeast

Rtg1 and Rtg3 are two basic helix-loop-helix (bHLH) transcription factors found in yeast Saccharomyces cerevisiae that are involved in the regulation of the mitochondrial retrograde (RTG) pathway. Under RTG response, anaplerotic synthesis of citrate is activated, consequently maintaining the supply of important precursors necessary for amino acid and nucleotide synthesis. Although the roles of ...

Activity of microRNAs and transcription factors in Gene Regulatory Networks: Activity of microRNAs and transcription factors in Gene Regulatory Networks

Yeast transcription factors.

Studies of yeast transcription factors have contributed greatly to understanding basic molecular mechanisms of eukaryotic gene regulation, largely due to powerful genetic approaches that are unavailable in other organisms. The broad outlines of these mechanisms are fairly well understood, and there is an increasing number of examples where detailed information is available.

Unraveling mechanisms that control platelet production.

Platelets are formed by giant precursor cells called megakaryocytes that reside within the bone marrow. The generation of platelets, and their release into the bloodstream by megakaryocytes, requires a complex series of remodeling events powered by the cytoskeleton to result in the release of many platelets from a single megakaryocyte. Abnormalities in this process can result in thrombocytopeni...